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. Author manuscript; available in PMC: 2018 Mar 13.
Published in final edited form as: Cell Rep. 2018 Feb 20;22(8):2118–2132. doi: 10.1016/j.celrep.2018.01.077

Figure 7. Stabilization of Ccnd1 and Tmem176b mRNAs by Bclaf1.

Figure 7

(A) Western blotting of immunoprecipitated Bclaf1 from differentiation day 3 cells.

(B) Relative expression levels of mRNAs in co-immunoprecipitated material with a Bclaf1 antibody. Results with and without reverse transcription are shown. Two PCR primer sets were used for Ccnd1, Ccnd2, and Tmem176b.

(C and D) Relative expression levels of three mRNAs in Bclaf1 KD (C) and Cry2 KD (D) cells treated with actinomycin D. The expression levels were normalized against Ctla2a mRNA at each time point and subsequently against 0 hr for each gene.

(E) Relative expression levels of mRNAs in co-immunoprecipitated material with a Bclaf1 antibody from Cry2 KD cells on day 3.

Data are presented as mean + or ± SD.