Figure 1. Massively Parallel snRNA-Seq Was Used to Define Cell Types in the Adult Mouse Spinal Cord.
(A) Summary of experimental strategy.
(B) Barnyard plot of pooled human and mouse spinal cord nuclei showing beads that were associated with human transcripts, mouse transcripts, both human and mouse transcripts (mixed), or those that could not be determined (undetermined).
(C) tSNE visualization plot of 17,354 spinal cord nuclei, colored according to seven major SC3-defined clusters: neurons, oligodendrocytes (oligos), meningeal and Schwann cells, astrocytes, vascular cells, oligodendrocyte precursor cells (OPCs), and microglia.
(D) Heatmap of normalized mean expression for key marker genes for each major SC3-defined cluster.