Skip to main content
. 2018 Jan 29;9(16):12732–12744. doi: 10.18632/oncotarget.24332

Figure 2. Affinity chromatography with the 10-7G mAb followed by Western blot analyses.

Figure 2

Western blot analyses with Hpt Ab and the 10-7G mAb and AAL blot analysis were performed for three fractions under affinity chromatography with the 10-7G mAb. Hpt products (A) and a sample consisting of a mixture of Hpt products and 1% fHpt-HCT purified from Hpt-transfected HCT116 cells (B) were used in chromatography. To detect Hpt α chain with Western blot, 15% SDS-PAGE was used. Silver staining was shown as loading control. Detail procedure was described in Materials and Methods.