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. 2018 Jan 11;9(16):12918–12931. doi: 10.18632/oncotarget.24161

Figure 6. Transcriptional regulation of CD147 by FOXC1.

Figure 6

(A) FOXC1 upregulated CD147 expression. HEK 293T cells were transfected with GV141-FOXC1 or FOXC1-siRNA, and the protein levels of CD147 in the transfected cells were detected using western blotting technique. (B) HEK 293T cells were cotransfected with the pGL3-Basic vector containing the human CD147 promoter (-1761/+37) and GV141-FOXC1, and the CD147 promoter activity was detected by the dual-luciferase reporter assay. (C) HEK 293T cells were cotransfected with different lengths of CD147 promoters and GV141-FOXC1, and the CD147 promoter activity was detected by the dual-luciferase reporter assay. (D) Schematic representation of the wild-type (Wt) and mutant (Mut) CD147 promoter regions. (E) HEK 293T cells were cotransfected with Wt CD147 or Mut CD147 promoters, and GV141-FOXC1, and the CD147 promoter activity was detected by the dual-luciferase reporter assay. (F) ChIP assay demonstrating the binding of FOXC1 to the CD147 promoter. Two percent of the lysate was used as the input control. **P < 0.01, ***P < 0.001.