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. 2018 Jan 12;9(16):12982–12994. doi: 10.18632/oncotarget.24204

Figure 4. Chelation of [Ca2+]i reversed the OA-induced activation of PI3K/Akt and promotion of HC11 proliferation.

Figure 4

(A) Effects of 100 μM OA and/or 2 μM BAPTA-AM on HC11 proliferation by using MTT analysis. (B) Western blot analysis of p-PI3K, PI3K, p-Akt, Akt, Cyclin D1, and p21 in HC11 after a 4-day culture in the presence of 100 μM OA and/or 2 μM BAPTA-AM. β-actin was used as the loading control. (C) Mean ± SEM of immunoblotting bands of p-PI3K/PI3K, p-Akt/Akt, Cyclin D1 and p21. The intensities of the bands were expressed as the arbitrary units. **P < 0.01 versus the Control group, ##P < 0.01 versus the 100 μM OA group.