Skip to main content
. 2018 Feb 14;9(16):13048–13059. doi: 10.18632/oncotarget.24495

Figure 1. Identification of miRNAs Targeting CXCL8.

Figure 1

(A) ELISA analysis of CXCL8 levels present in the conditioned media of stromal fibroblasts isolated from normal (NL, blue, N = 8), colitic (CT, red; N = 9) and cancerous colons (CA, orange; N = 5). See Supplementary Table 2 for patient demographics. (B) Screening of a Miridian human mimic miRNA library using a pMirGLO-CXCL8 3′UTR luciferase construct in primary colitic fibroblasts. Green dots represent luciferase levels from the individual miRNA mimics, red pluses those of a siRNA targeting the 3′UTR of CXCL8 and the black pluses those of a non-targeting negative control. Dashed black line delineates separation between mimics and the negative controls. (C) Pathway analysis of the identified miRNAs. Individual pathways and their associated miRNAs are depicted in the individual circles. miRNAs identified in these processes and selected for subsequent analysis are indicated in the center. (D) q-RT-PCR analysis of the miRNAs identified in (C) for their expression in multiple stromal fibroblasts. (NL: N = 3; CT: N = 3, CA: N = 2).