Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 14;9(16):13048–13059. doi: 10.18632/oncotarget.24495

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2018 Signs et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

(A) Primary fibroblasts derived from normal, colitic and cancerous stroma were analyzed for the expression levels of the primary transcript encoding miR-20a-5p (miR20HG) and its mature form were quantified by mRNAseq and qRT-PCR, respectively. (B, C) qRT-PCR for the pre-miR-20a-5p (B) and miR-20a-5p (C) comparing normal and colitis-associated fibroblasts. Expression was normalized to U18 RNA. (D) Expression of c-MYC by mRNAseq. (E) Levels of CXCL8 mRNA and protein by mRNAseq and ELISA. Statistical significance in (A–E) was determined using t-test; asterisks indicate significance (^*p < 0.05 and ^**p < 0.01). (F) Spearman’s correlation of miR-20a expression vs. secreted CXCL8 revealing a statistically significant inverse monotonic relationship (p < 0.001; r_s = –0.654). Normal colon fibroblasts: black, colitic colon fibroblasts: red, cancer-associated fibroblasts: green.