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Localization of KIM-1 protein in lipid microdomains in HK-2 cells. Membrane fractions were prepared from the HK-2 cells using non-detergent sodium carbonate extraction and sucrose density gradient separation. Twenty-five microliter of each fraction (1–12) was added on SDS-PAGE gels and proteins were detected by Western blot. KIM-1 was colocalized with caveolin-1 and clathrin to the lipid raft domains.
