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. 2018 Feb 16;31:2058738418759180. doi: 10.1177/2058738418759180

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Effects of APS on LPS-induced the expression and release of inflammatory cytokines in H9c2 cells. (a)–(b) The mRNA and protein expression levels of IL-6, IL-8, and TNF-α in H9c2 cells were measured by qRT-PCR and western blot after treatment with LPS or co-treatment with APS and LPS. (c)–(e) The release of IL-6, IL-8, and TNF-α was measured by ELISA assay in H9c2 cells. Different letters above the bars (a, b, c, d, e) indicate that the means of different groups were significantly different (P < 0.05) by ANOVA. Each experiment was repeated at least three times.

APS: Astragalus polysaccharide; LPS: lipopolysaccharide; IL-6: interleukin-6; IL-8: interleukin-8; TNF-α: tumor necrosis factor-alpha; qRT-PCR: quantitative real-time polymerase chain reaction; ELISA: enzyme-linked immunosorbent assay; ANOVA: one-way analysis of variance.

***P < 0.001 vs control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs LPS group.