Fig 4. Normal basal and differentiated airway epithelial cells differ in gene expression patterns after mechanical injury.

A. Volcano plots in AEC from asthmatic donor lungs to demonstrate differential expression in probes in the resting (without mechanical injury) state (N = 6 in each group) between differentiated (dAEC) and basal AEC, using all expressed gene probe sets (n = 41,733) as an input dataset. For each, the number of up- and down-regulated probes (≥1.5 fold change, vertical dashed lines, adjusted P < 0.05, horizontal dashed line) is provided. B. Differential expression for selected gene probes involved in inflammation and inflammation signaling at 2–24 hr after injury versus non-injured cells is provided as a heat map.