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. 2018 Mar 13;13(3):e0194163. doi: 10.1371/journal.pone.0194163

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© 2018 Motoyama et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Morphometric analysis of Sirius red-positive (A) and α-SMA-positive (B) areas in SVR patients, with and without HCC. Positive areas for Sirius red and α-SMA immunohistochemistry were determined using the analysis application BZ-H3A (Keyence, Osaka, Japan). Note that both the Sirius red- and α-SMA-positive areas were not improved in the SVR-HCC group. CYGB and α-SMA-positive cells were counted under a × 100 objective. Note that the CYGB and α-SMA-positive cells numbers remained in the SVR-HCC group; * p < 0.05; **, p < 0.01.