Fig 4. Impairment of Rab5 and Rab11 abundance and localization in dlg1 mutant clones is Atf3-dependent.

(A-D) eyFLP-mediated mitotic recombination combined with a co-expression of p35 was used to generate larger clones (GFP) in the EADs of the indicated genotypes. The levels and localization of early Rab5-positive endosomes are disturbed upon loss of dlg1 both posterior (A’) and anterior (B’) to the morphogenetic furrow, while recycling Rab11 vesicles are enriched in dlg1 mutant clones relative to surrounding tissue (A”,B”). Both Rab5 and Rab11 vesicle amounts and distribution are normalized in atf3⁷⁶dlg1^G0342 double mutant clones (C-D). On the cross sections, blue arrowheads indicate the regular Rab5 enrichment along the photoreceptors (A’,C’), yellow arrowheads indicate the apical localization of Rab5 in epithelial cells anterior of the MF (B’,D’). Note that both patterns are lost in dlg1 deficient cells. Discs were counterstained with DAPI. White arrows indicate cross sections, which appear below the corresponding panels and are oriented apical side up. Clones are outlined by white dotted lines. All images show EAD 7 days after egg laying. Scale bars: 10 μm (A,C), 20 μm (B,D).