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. 2018 Mar 8;13:1361–1379. doi: 10.2147/IJN.S157000

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© 2018 Kelley et al. This work is published and licensed by Dove Medical Press Limited

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RK-GFP DNA was detected in the retina and RPE.

Notes: Retinas and RPE were harvested from animals subretinally (two-site or one-site) or intravitreally injected with NP RK-GFP or saline. (A) Expression of GFP message as measured by qRT-PCR in the four quadrants of eyes receiving two-site subretinal injections (triangle), one-site subretinal injections (circle), or intravitreal injections (square). Colors correspond to individual eyes and are the same in (B). Black lines indicate mean. (B) Relative levels of RK-GFP DNA were measured in injected eyes by qPCR in the retina (closed symbols) and RPE (open symbols). Black bar indicates mean. Legend indicates animal #/eye.

Abbreviations: IV, intravitreal injection; SR, subretinal injection; NP, nanoparticle; qPCR, quantitative polymerase chain reaction; qRT-PCR, quantitative reverse transcription polymerase chain reaction; RPE, retinal pigment epithelium.