Table 3.
In vitro effects of various MWs and concentrations of CS NPs on the mortality rate of T. gondii RH strain tachyzoites using MTT assay
| MW of CS NPs | Concentration | Time (min)
|
P-value | |||
|---|---|---|---|---|---|---|
| 30
|
60
|
120
|
180
|
|||
| Mean mortality ± SD | Mean mortality ± SD | Mean mortality ± SD | Mean mortality ± SD | |||
| LMW | 500 ppm (0.05%) | 27.9 ± 1.2 | 45.1 ± 1.4 | 78.8 ± 3 | 100 ± 0.0 | 0.023* |
| MMW | 25.9 ± 1.4 | 37.1 ± 2.5 | 81.1 ± 3.1 | 98.7 ± 1.1 | ||
| HMW | 24.4 ± 1.7 | 37.3 ± 2.5 | 54 ± 2.7 | 69.2 ± 3 | ||
| LMW | 1,000 ppm (0.1%) | 45.3 ± 2.8 | 60.3 ± 1.9 | 87.1 ± 2.7 | 100 ± 0.0 | 0.006* |
| MMW | 28 ± 1.9 | 46.7 ± 2.2 | 83.3 ± 2.9 | 100 ± 0.0 | ||
| HMW | 26.7 ± 2.1 | 48.8 ± 1.2 | 71.8 ± 1.9 | 84 ± 2.1 | ||
| LMW | 2,000 ppm (0.2%) | 62.8 ± 3.3 | 81.37 ± 1.3 | 98.3 ± 1.5 | 100 ± 0.0 | 0.002* |
| MMW | 30.1 ± 1 | 52.2 ± 2.3 | 85.9 ± 2.2 | 100 ± 0.0 | ||
| HMW | 29.1 ± 2.5 | 50.8 ± 2.3 | 86.4 ± 1.5 | 100 ± 0.0 | ||
| NC | 0.0 ± 0.0 | 0.0 ± 0.0 | 4.6 ± 0.5 | 8 ± 2 | ||
Notes: Values are given as the mean ± SD;
*
Significant at P < 0.05; NC, negative control of normal saline containing 1% (w/v) acetic acid.
Abbreviations: MWs, molecular weights; CS NPs, chitosan nanoparticles; LMW, low molecular weight; MMW, medium molecular weight; HMW, high molecular weight; NC, negative control; SD, standard deviation; T. gondii, Toxoplasma gondii.