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. 2017 Dec 26;142(7):1490–1502. doi: 10.1002/ijc.31170

Figure 5.

Figure 5

BCT‐100‐induced cell cycle arrest leads to necrotic cell death. (a) ALL cell lines were cultured with 600 ng/mL BCT‐100. Cell cycle analysis was performed after 72 hrs. BCT‐100 increases the percentage of cells in G0/G1 arrest. (b) Relative expression of cyclins A, B1, D, B2 and E1 in BCT‐100‐treated ALL patients' blasts compared with untreated controls (hashed line) were investigated by qPCR. Representative data of 6 patients are shown. (c) ALL blasts from patients were treated with BCT‐100 (600 ng/mL). Analysis of cell death was performed by transmission electron microscopy. Representative micrographs of three of six patients were shown. Upper panel: untreated cells. Lower panels: post treatment with 600 ng/mL BCT‐100. Features consistent with organelle enlargement, cell membrane permeablisation and cellular fragmentation with 600 ng/mL BCt‐100. Experiments performed on six separate occasions.