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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Hepatology. 2018 Mar 24;67(5):1710–1725. doi: 10.1002/hep.29522

Figure 2.

Figure 2

Variants of LAP2 found in NAFLD patients interfere with binding to lamin A. (A) Huh7 cells were co-transfected with myc-tagged wild-type (WT) or variant LAP2α and GFP-tagged lamin A, followed by immunoprecipitation using an antibody directed to the GFP tag. Immunoprecipitates were resolved on SDS-PAGE, and precipitated proteins were visualized after immunoblotting with antibodies to myc or the GFP tag. (B) Huh7 cells were co-transfected with GFP-tagged lamin A and WT LAP2α or its truncated variant (LAP2 1-99). Lamin A was immunoprecipitated, followed by visualization as in panel A. Ig, immunoglobulin; M.W., apparent molecular weight; kDa, kilodaltons; IP, immunoprecipitation; IB, immunoblot. Arrowhead indicates a non-specific band recognized by the myc antibody.