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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Hepatology. 2018 Mar 24;67(5):1710–1725. doi: 10.1002/hep.29522

Figure 3.

Figure 3

The truncated variant of LAP2 is mislocalized in transfected cells. (A) Huh7 cells were transfected with full-length (F.L.) LAP2α or truncated LAP2 (1-99) with DDK tag, or empty vector. Cells were then fixed, and LAP2 was visualized by indirect immunofluorescence using anti-FLAG antibody (which recognizes the DDK tag). Scale bar: 50 μm (lower magnification images: first, second, and fourth rows), 20 μm (higher magnification images: third and fifth rows). (B) Cells transfected as in panel A were scored according to whether LAP2 was specifically localized to the nucleus or mislocalized throughout the cell (represented as percent of transfected cells with mislocalized LAP2). Data were derived from counting 9–12 high-power fields from three independent experiments. Error bars represent the standard error of the mean. Student’s t test was used to determine statistical significance at a threshold of P<0.05.