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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Hepatology. 2018 Mar 24;67(5):1710–1725. doi: 10.1002/hep.29522

Figure 4.

Figure 4

Truncated LAP2 causes increased lipid accumulation in transfected cells. (A) Huh7 cells were transfected with DDK-tagged full-length (F.L.) LAP2α or truncated LAP2 (1-99) or empty vector, then treated with 500 μM oleic acid or vehicle (isopropanol) in serum-free medium overnight. After fixation, transfected LAP2 was visualized by indirect immunofluorescence using anti-FLAG antibody. Lipid droplets were stained with BODIPY 493/593 as described in Materials and Methods. Representative images are shown for each condition; scale bar, 20 μm. (B) Lipid accumulation was quantitated as described in Materials and Methods for >10 high-power fields for each condition, and the data shown are representative of 3 independent experiments. Error bars represent standard error of the mean. Student’s t test was used to determine statistical significance at a threshold of P<0.05.