Table 4.

Primary versus recurrent GBM sample continuous variables.

Variable	Group	n	Mean (yrs or % positive staining)	SD	p value
Age	Primary	38	61.05	10.67	0.0009
	Recurrent	11	43.36	12.71
CD3	Primary	37	0.35	0.44	0.9416
	Recurrent	11	0.33	0.35
CD8	Primary	36	0.12	0.14	0.1937
	Recurrent	11	0.30	0.44
CD68	Primary	38	5.20	3.39	0.7120
	Recurrent	11	5.46	2.06
CD163	Primary	38	6.12	6.40	0.7658
	Recurrent	11	5.56	3.72
FOXP3	Primary	37	0.35	0.47	0.9279
	Recurrent	10	0.37	0.47
PDL1	Primary	37	8.11	7.66	0.4508
	Recurrent	11	7.42	4.34
CTLA4	Primary	38	0.43	0.59	0.9525
	Recurrent	11	0.27	0.23
CD70	Primary	34	0.69	1.38	0.3401
	Recurrent	6	0.82	0.79
PD1	Primary	38	1.42 × 105	3.95 × 105	0.4888
	Recurrent	11	0.38 × 105	0.75 × 105

The patient age and percent positive cell expression of immune markers are shown in primary and recurrent samples. The primary samples were associated with older patients compared to recurrent samples. No significant differences in immune marker expression was found between primary and recurrent samples.