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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Anal Biochem. 2018 Jan 12;545:4–12. doi: 10.1016/j.ab.2018.01.008

Fig. 8.

Fig. 8

Underwater real-time RPA amplification of Listeria DNA. Samples from left to right: 2.4×108 Listeria CFU/mL, 2.4×107 Listeria CFU/mL, 2.4×106 Listeria CFU/mL, and Salmonella DNA as negative control. These cell phone images were taken over a period of 15 min. The images show that RPA amplification in the thermos can be monitored in real time using a smartphone above the oil layer inside the thermos. No active temperature control was needed. Since the oil layer is transparent and can act as a lid for the reaction chamber, the real-time RPA progression can also be monitored by the naked eye when an appropriate orange filter for emission viewing is used by the viewer.