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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Anal Biochem. 2018 Mar 15;545:72–77. doi: 10.1016/j.ab.2018.01.018

Fig. 3.

Fig. 3

Quantification of methylation stoichiometry in protein standards. (A) Coomassie-blue stained SDS-PAGE gel of input protein standards calmodulin (lane 1, 1 μg), MBP (lane 2, 2 μg) and mixed histones (lane 3, 3 μg). Bands excised for hydrolysis and LC-MS/MS analysis are marked by asterisks. The excised histone bands consisted of core histones H2A, H2B and H3. (B) Methylation stoichiometry determined by LC-MS/MS and eqns. 1–4. Bar height indicates contributions of 1meK, 2meK, 3meK, 1meR and 2meR to mol methyl/mol protein in each sample (triplicate determination) whereas error bars represent the standard deviation.