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. Author manuscript; available in PMC: 2019 Mar 12.
Published in final edited form as: Dev Cell. 2018 Mar 1;44(5):624–633.e4. doi: 10.1016/j.devcel.2018.01.024

Figure 3.

Figure 3

The maintenance of growth and pattern formation for crypt foci is regulated by WNT and BMP. (A) Crypts were maintained for 2 days under a matrix of WNT3a vs. BMP4 concentrations in ENR media (see Experimental Procedures). Cells were labeled with EdU for two hours before fixation and staining for EdU and DNA. (B,C) As in (A), but crypt number (B) and average number of cells per crypt (C) were quantified. (D, E, F) As in (A–C) but with 5 days of growth factor treatment. Scale bars = 25 μM. See also Figure S3.