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. 2018 Mar 13;8:4453. doi: 10.1038/s41598-018-22737-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of cytochalasin D delivery on optic nerve head and retinal ganglion cell layer nuclear counts. (A) Nuclear density within the anterior optic nerve head (ONH; 5 µm sections; 0–100 µm posterior to Bruch’s membrane) after sham (n = 4) and cytochalasin D (Cyt D; n = 7) delivery to the optic nerve (no statistically significant difference was noted between groups). (B) Nuclear density within the retinal ganglion cell (RGC) layer of the superior peripapillary retina (5 µm sections; 0–250 µm from the superior optic nerve head) after sham (n = 4) and Cyt D (n = 6) delivery to the optic nerve (no statistically significant difference was noted between groups). Error bars indicate standard error of the mean.