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. 2018 Mar 13;8:4453. doi: 10.1038/s41598-018-22737-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of cytochalasin D delivery on optic nerve head axonal microtubule levels. (A–C) Normal optic nerve head (ONH) labeled with fluorescent-tagged phalloidin (F-actin maker) and axon-specific anti-βIII tubulin antibodies. (D,E) ONH tubulin levels after sham (pledget soaked in vehicle only) delivery and 1 mM Cyt D delivery to the junction of the superior optic nerve and globe. (F) ONH tubulin content assessed by fluorescence intensity measurement of anti-βIII tubulin antibody labeling after sham (n = 4) and 1 mM Cyt D delivery (n = 6) to the optic nerve (no statistically significant difference was noted between groups). Error bars indicate standard error of the mean. a.u. = arbitrary units; Cyt D = cytochalasin D; F-actin = filamentous actin; Inf. = inferior; ONH = optic nerve head; Sup. = superior.