Table 2.
Characteristics of the proteins used to characterize permeability cut-off and observed transport rates.
| Analyte | Size | Charge | Transport rate | ||||||
|---|---|---|---|---|---|---|---|---|---|
| MW | RHa | IEPb | charge at pH | (g m−2 h−1) | |||||
| (kDa) | (nm) | (pH) | 5 | 7 | 9 | pH 5 | pH 7 | pH 9 | |
| Cytochrome c | 12.5 | 1.78 | 10.04 | + | + | +/0 | 0.38 | 0.58 | 0.54 |
| Myoglobin | 16.7 | 2.12 | 6.85/7.33 | + | +/0 | — | 0.27 | 0.42 | 0.51 |
| Bovine serum albumin | 66 | 5.15 | 4.9 | 0 | — | — | 0.04 | 0.09 | 0.05 |
| Alcohol dehydrogenase | 150 | 4.55 | 5.4–5.8 | +/0 | — | — | n.d. | 0.99 | 1.59 |
| Apoferritin | 443 | 6.73 | 4 | — | — | — | n.d. | 0 | 0 |
| Thyroglobulin | 670 | 8.58 | 4.5–5.0 | 0/— | — | — | 0 | 0 | 0 |
| Blue Dextran | 2000 | 26.89 | 7 | + | 0 | — | 0 | 0 | 0 |
n.d.: 1 g L−1 protein solutions were not used for diffusion experiments due to aggregation.
a,b Values for the hydrodynamic radii (RH) and the isoelectric points (IEP) were taken from the literature as listed in Supplementary Table 2.