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. 2018 Mar 13;8:4433. doi: 10.1038/s41598-018-22806-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Purity evaluation of nicotinamide mononucleotide (NMN) separated by SEC PolyHEA using reversed-phase HPLC (C18). The light absorption spectrum recorded by the Jasco MD2015 UV-Vis Photodiode Array (PDA) detector during HPLC isocratic elution, NMN standard Sigma (however, impure as shown) (A,C) versus NMN purified from bacterial lysate by SEC PolyHEA (B,D). HPLC Chromatogram 260 nm isocratic elution of NMN standard Sigma 95% purity (E) versus NMN purified from the bacterial lysate by SEC PolyHEA (F). Mobile phase: 10% acetonitrile, flow rate 0.9 mL/min, column: Genesis C18 James Column 250 × 4.6 mm, 4 µm, 120 Å, injected sample: 20 μL. 10 mM NMN, starts eluting at 3 minutes.