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. 2018 Mar 13;8:4433. doi: 10.1038/s41598-018-22806-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The schematic diagram for the proposed nicotinamide mononucleotide (NMN) purification process. Bacterial cells are grown in the bioreactor, the media containing bacteria is pumped into a separating funnel and stored at 4 °C. After 24 hours, the sedimented bacterial cells are collected, cell membranes are disrupted by ultrasound on ice, cell debris and macromolecules are separated by ultracentrifugation and discarded, NMN is finally separated by a single step size exclusion chromatography (SEC) on a PolyHEA column eluted with 50 mM formic acid.