Figure 3.

Comparing the different extraction and identification methods. The exosome RNA were extracted by 4 different methods and identificated by poly A tail or stem loop method, respectively. (A) The purity of Exosome RNA were extracted by 4 different methods, and group D was extracted by exoRNeasy Serum/Plasma Midi Kit compared with the other groups, the difference was statistically significant (p < 0.05); (B) The Ct values of miR-27a-3p and miR-16 were detected by poly A tail or stem loop method.