. 2018 Feb 23;410(9):2343–2361. doi: 10.1007/s00216-018-0889-x

Table 5.

Metabolic reactions observed for the investigated thiosemicarbazones via LC-HRMS after cell-free incubations with HLM with the intensities of the obtained metabolites (✓: > 10⁶ AU, ~: 10⁵–10⁶ AU, x: < 10⁴ AU)

Modification	Metabolic reaction	1	2	3	4	5	6	7	8	9	10
[M–2H + H]⁺	Dehydrogenation	✓	✓	✓	✓	✓	✓	x	✓	x	✓
[M + O + H]⁺	Hydroxylation	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
[M + O–2H + H]⁺	Hydroxylation/Dehydrogenation	✓	✓	✓	✓	✓	~	x	✓	x	✓
[M–S + H]⁺	Desulfuration C (Amidrazone)	✓	~	✓	✓	✓	✓	✓	✓	✓	✓
[M–S–2H + H]⁺	Desulfuration/Dehydrogenation	✓	x	✓	✓	~	✓	✓	x	~	✓
[M–S + O + H]⁺	Desulfuration C=O (Semicarbazone)	✓	~	~	✓	✓	✓	✓	~	✓	✓
[M–S + O–2H + H]⁺	Desulfuration/Dehydrogenation	✓	~	~	~	✓	~	x	~	x	✓