Fig. 5.

Functional characterization of significantly regulated phosphorylation sites. a iceLogos of the SAM-upregulated phosphorylation sites from Fig. 3c vs. the respective non-regulated sites as background. The iceLogos show the ATM/ATR kinase substrate [s/t]Q motif significantly enriched for all tested quantification approaches. b, c Heat maps showing b a kinase motif and c GO-term enrichment of significantly SAM-up/downregulated phosphorylation sites from Fig. 3c vs. the respective non-regulated sites as background. Enrichment was performed using Fisher exact tests within Perseus with relative enrichment on gene level and an FDR of 0.02. The numbers above the heatmap show the total number of enriched motifs/GO-terms, while the heat maps below show b the most significantly regulated motifs or c all GO-terms with “damage”, “repair”, “checkpoint”, “cell cycle”, or “chromosome”, indicative of an activated DDR, respectively