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. 2018 Mar 13;9:1051. doi: 10.1038/s41467-018-03146-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Detection of fHbp and PorA by ChA antisera. a Immunisation strategy. Mice (n = 8 per group) were subcutaneously immunised three times with each combination of ChA/Adjuvant. b, d Western blots of N. meningitidis whole-cell lysates probed with each set of ChA/adjuvant antisera: b detection of fHbp, d detection of PorA, complete western blots are in Supplementary Figures 6 and 7. c, e Flow cytometry analysis showing binding of each set of ChA/adjuvant antisera to N. meningitidis H44/76 strains WT, ∆fHbp, ∆porA and ∆fHbp∆porA. s.d. of independent assays (n = 3) is indicated. Two-way ANOVA and Dunnett’s method of multiple comparison were used to compare the fluorescence intensity of ChA antisera with PBS control sera (c, e *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001)