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. 2018 Mar 13;9:1051. doi: 10.1038/s41467-018-03146-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Immunogenicity of ChAs. Titres from SBAs performed with N. meningitidis strains H44/76 (a, n = 3), H44/76∆porA (b, n = 3), and H44/76∆fHbp (c, n = 2) and ChA/adjuvant antisera. SBAs with H44/76 and H44/76∆porA were conducted using pooled ChA/adjuvant antisera, and SBAs with H44/76∆fHbp were conducted with ChA/adjuvant antisera from individual mice. Geometric mean and s.d. of independent assays (n > 2) are indicated. Flow cytometry was used to detect binding of mouse isotypes IgG1, IgG2a, IgG2b, IgG3 and IgM in ChA antisera to N. meningitidis strains H44/76∆porA (d, f) and H44/76∆fHbp (e, g). Mouse isotypes binding to N. meningitidis were detected with isotype specific secondary antibodies; s.d. of independent assays (n = 3) is indicated. Two-way ANOVA and Dunnett’s method of multiple comparison were used to compare SBA titres from pooled antisera (a, b) and ChA antisera to PBS control sera (d–g) (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001)