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. 2018 Mar 13;8:4407. doi: 10.1038/s41598-018-22729-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detergent solubilisation and thermal stability of mGlu₅-Δ856 [³H]-MPEP-bound receptor in insect cells. (A) Quantification of unpurified mGlu₅-Δ856 receptor solubilized at 4 °C using [³H]-MPEP binding assay measured in a series of maltoside-based detergents including lauryl maltose neopentyl glycol-3 (MNG3), n-Dodecyl-β-D-Maltoside (DDM) and n-Decyl-β-D-Maltoside (DM) supplemented with different ratio of cholesteryl hemisuccinate (CHS). (B) Thermal stability of the mGlu₅-Δ856 [³H]-MPEP-bound receptor solubilized in four different conditions; DDM (0.83%), DDM-CHS (0.83–0.083%), MNG3 (0.83%) and MNG3-CHS (0.83–0.083%). Data points for both assays (A,B) represent the mean ± SEM of three independent experiments. Dunnett’s test as part of one-way ANOVA was used for comparison with MNG3-CHS (0.83–0) and DDM-CHS (0.83–0) set as reference level for MNG3 and DDM supplemented with CHS, respectively.