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. 2018 Mar 13;8:4469. doi: 10.1038/s41598-018-22777-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of agar concentration on the number of eggs laid. At each concentration, six replicated populations (grey diamonds) of 100 D. simulans each laying eggs for 12 hours and eggs were counted manually. Black bars depict medians. No significant difference in egg counts was detected between recipes at 2% and 3%, all other comparisons were significantly different at P < 0.05 (GLMM followed by Tukey’s HSD test, see main text for P-values).