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. 2018 Mar 13;8:4395. doi: 10.1038/s41598-018-22662-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TSA suppressed activation of primary rat HSCs. (A) Primary cultured rat HSCs were auto-activated during culture. This was confirmed by fluorescence image, immunofluorescent staining and oil red O staining. (B) Western blot analysis showed that the protein levels of collagen type I and α-SMA in day 10 HSCs were increased compared to day 3 cells. **p < 0.01 compared to day 3 group. (C) Expression of collagen type I, α-SMA and C/EBP-α in day 10 HSCs after treatment with TSA. *p < 0.05 and **p < 0.01 compared to no treatment group.