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. 2018 Mar 13;9:1057. doi: 10.1038/s41467-018-03476-6

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© The Author(s) 2018, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — GNA13 promotes cell migration in ATRX-mutant GSCs. a, b western blot (a) and RT-qPCR (b; three replicates) showing increased GNA13 expression in IDH1-mutant (IDH1 R132H), ATRX-deficient GSC line (GS 5–22). c transwell migration assay (5 replicates) demonstrating increased motility in IDH1-mutant, ATRX-deficient GSC line (GS 5-22) relative to IDH1-mutant, ATRX-intact GSC line (TS 603). d–e western blot (d) and RT-qPCR (e; three replicates) showing decreased Gna13 expression upon transient transfection of Atrx- mNPCs with human ATRX (hATRX) relative to control vector (Con). f transwell migration assay (5 replicates) demonstrating decreased motility in hATRX transfected, Atrx- mNPCs. h–i western blot (h) and RT-qPCR (i; three replicates) showing decreased GNA13 expression upon transient transfection of GS 5-22 cells with hATRX relative to Con. j transwell migration assay (5 replicates) demonstrating decreased motility in hATRX transfected GS 5-22 cells. All western blots used Vinculin as a loading control. In all cases, error bars reflect standard deviation and P values determined by unpaired two-tailed t-test