Table 1.

Cell culture conditions during proliferation and maturation of HCEC.

Proliferation medium (P)	Maturation medium (M)
P	n/a
P + TGF-β1 2 ng/ml	n/a
P + TGF-β1 2 ng/ml + SB431542 10 µM	n/a
P + TGF-β1 2 ng/ml + AG-1478 1 µM	n/a
P	P
P	M
P	M + TGF-β1 2 ng/ml
P	M + TGF-β1 2 ng/ml + SB431542 10 μM
P	M + TGFβ1 2 ng/ml + EGF 5 ng/ml
P	M + TGFβ1 2 ng/ml + EGF 5 ng/ml + AG-1478 1 µM
P	M + TGFβ1 2 ng/ml (1–7 DPC) + M (8–14 DPC)

DPC: days post-confluency; EGF: epidermal growth factor; HCECs: human corneal endothelial cells; TGF-β1: transforming growth factor β-1.