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. 2018 Mar 7;9:201. doi: 10.3389/fphar.2018.00201

FIGURE 2.

FIGURE 2

Mangiferin administration decreased hepatic lipid accumulation via SIRT1-AMPK activation. (A) Separation of hepatic lipid by thin-layer chromatography (TLC) analysis (A1), and semi-quantification analysis by ImageJ software (A2). Gray-scale values were represented by % of the total lipid from three separate mice in each group. (B) Protein expression of Sirt1 (B1) was suppressed but restored by MGF in a dose-dependent manner. Densitometric quantification of the protein expressions was measured by ImageJ software (B2). Values are means ± SEM, ∗∗p < 0.01, ∗p < 0.05 versus DC group. (C) AMP-activated protein kinase (AMPK) activity (C1) and liver kinase B1 (LKB1) activity (C2) were suppressed but restored by MGF in a dose-dependent manner. Densitometric quantification of the protein expressions was measured by ImageJ software (C3). Values are means ± SEM, ∗∗p < 0.01, ∗p < 0.05 versus DC group. (D) Expression of p-AMPK in the liver determined by immunohistochemical staining (D1) and quantitative analyzed by Histoscore system (D2). Values are means ± SEM.