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. 2017 Sep 20;96(12):4409–4418. doi: 10.3382/ps/pex247

Figure 4.

Figure 4.

Effects of insulin immune-neutralization and fasting on hypothalamic levels of mRNA for genes involved in glucose transport and metabolism. (A) PCR products for glucose transporters 1, 2, 3, and 8 (GLUT1, GLUT 2, GLUT3, and GLUT8, respectively). Hypothalamic levels of mRNA for (B) GLUT1, (C) GLUT2, (D) GLUT3, and (F) GCK were determined by reverse transcription-quantitative PCR. (E) PCR products for glucokinase (GCK). Numbers 1 to 6 in panels A and E refer to different tissues. Samples are identified as follows: 1, C_5; 2, AI_5; 3, no RT sample; 4, brain sample from 3 wk old chicken; 5 liver sample from embryonic day 18 and 6, blank. See legend to Figure 1 for further details.