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. 2018 Jan 22;7(2):e005696. doi: 10.1161/JAHA.117.005696

Figure 5.

Figure 5

A disintegrin and metalloprotease‐22 (ADAM22) inhibits the protein kinase B (AKT) activation on hypertrophic stresses. A, Western blot analysis of the mitogen‐activated protein kinase expression and phosphorylation in the indicated groups (n=6 mice per group). B, Western blot analysis of the AKT, glycogen synthetase kinase (GSK) 3β, mammalian target of rapamycin (mTOR), and p70S6K expression and phosphorylation in indicated groups (n=5 mice per group). C, Western blot analysis of the AKT, mTOR, GSK3β, and p70S6K expression and phosphorylation in the indicated groups (n=5 samples per group). Data are representative images or present as the mean±SD of each group from at least 3 independent experiments. Statistical analysis was performed by 1‐way analysis of variance and post hoc tests. ERK indicates extracellular signal–regulated kinase; JNK, c‐Jun N‐terminal kinase; MEK, mitogen‐activated protein/ERK; NS, nonsignificant; and TAC, transverse aortic constriction. *P<0.05 vs ADAM22‐Flox or nontransgenic/sham group, † P<0.05 vs ADAM22‐Flox or nontransgenic/TAC 4‐week group, ‡ P<0.05 vs AdshRNA or AdGFP/PBS group, § P<0.05 vs AdshRNA or AdGFP/AngII group.