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. 2018 Jan 22;7(2):e006727. doi: 10.1161/JAHA.117.006727

Figure 4.

Figure 4

Assessment of swine immune response to hAPC. A through F, Humoral immune response to hAPCs. hAPCs were incubated with swine or human serum (negative control) at different dilutions. There was no difference in the binding of anti‐swine IgM or IgG antibodies on hAPCs when incubated with human serum (A and C) or swine serum (B and D). Similarly, no difference was observed with regard to the levels of C3b, a metabolite of complement cascade (E and F). Data are expressed as mean±SEM; N=3 biological replicates. G and H, Cytotoxic activity of swine splenocytes on hAPCs. To investigate cell‐mediated immune response, hAPCs (target cells) were incubated with interleukin‐activated swine splenocytes (effector cells). G, Representative flow cytometry graphs of hAPCs challenged with increasing concentrations of swine splenocytes. Values in each quadrant represent the percent of total cells. H, Bar graphs show the percent of necrotic (Annexin V−/P.I.+), late apoptotic (Annexin V+/P.I.+), early apoptotic (Annexin V+/P.I.−), and viable (Annexin V−/P.I.−) hAPCs. Data are expressed as mean±SEM; N=2 biological replicates; *P<0.05 vs the “0:1” group. A.U. indicates Arbitrary Units; FITC, Fluorescein isothiocyanate; hAPCs, human adventitial pericytes; P.I., propidium iodide.