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. 2018 Feb 8;7(4):e008187. doi: 10.1161/JAHA.117.008187

Figure 6.

Figure 6

Systemic and local inflammation is diminished in neutrophil elastase−/−/Apolipoprotein E−/− double knockout (NE_KO) mice fed a high‐fat diet (HFD) for 12 weeks. Both wild‐type (WT) and NE_KO male mice were fed an HFD for 12 weeks. Plasma (A and B) and aortas (C) were harvested and subjected to plasma lipid (A) and inflammatory cytokine (B) profile assay, as well as real‐time quantitative polymerase chain reaction analyses to detect aortic (local) expression of inflammatory genes as indicated (C), respectively. Quantitative data (mean±SEM) for both plasma lipid and inflammatory cytokine levels from 12 WT and 11 NE_KO mice are presented. *P<0.05 (vs WT mice, unpaired t test). Data in C were aortic mRNA expression levels from 5 mice (n=5 mice per group) presented as median with interquartile range. *P<0.05 (vs WT mice, Mann–Whitney U Test). HDL‐C indicates high‐density lipoprotein cholesterol; IFN‐γ, interferon γ; IL, interleukin; LDL‐C, low‐density lipoprotein cholesterol; MCP‐1, monocyte chemoattractant protein‐1; TC, total cholesterol; TG, triglyceride; TNFα, tumor necrosis factor α.