Figure 6.

Ca_v1.2 L‐type Ca²⁺ channel protein is unchanged in Lrrc10 ^−/− mice and associates with LRRC10 in wild‐type (WT) mouse hearts. A, Lysates were prepared from WT and Lrrc10 ^−/− mouse hearts (n=4 each group), and 100 µg of input protein was loaded. Representative immunoblot shows expression of Ca_v1.2 channel protein and GAPDH loading control in WT and Lrrc10 ^−/− lysates. Only LRRC10 (leucine‐rich repeat–containing 10) protein is expressed in WT, not Lrrc10 ^−/− lysates. Normalized Ca_v1.2 protein signal (Ca_v1.2/GAPDH) was analyzed by Student t test and found not significantly different between WT and Lrrc10 ^−/−. B, Representative immunoblot of WT and Lrrc10 ^−/− mouse heart lysates immunoprecipitated with anti‐LRRC10 or anti‐IgG control antibodies and immunoblotted with anti‐Ca_v1.2 or anti‐LRRC10 antibodies with the arrows indicating Ca_v1.2 and LRRC10, respectively. The immunoblot is representative of 3 independent experiments. *IgG heavy and light chains, which are ≈52 and 30 kDa, respectively.