Table 1.

Summary of Observed Mutations and Fitnesses.

Genotype	Nucleotide	Change	Gene	Residue	Change	No. of Occurences	wa	SE	sb
ID11	2534	G → T	J	20	V → L	1	20.1	0.32	0.30
	3665	C → T	F	355	P → S	5	19.7	0.21	0.28
	3850	G → A	F	416	M → I	3	19.1	0.40	0.24
	3857	A → G	F	419	T → A	1	18.8	0.41	0.22
	3567	A → G	F	322	N → S	1	18.3	0.42	0.19
	2520	C → T	J	15	A → V	6	18.2	0.45	0.18
	3543	C → T	F	314	A → V	1	17.6	0.37	0.14
	3864	A → G	F	421	D → G	1	17.3	0.39	0.13
	2609	G → T	F	3	V → F	1	17.1	0.39	0.11
ID8	4496	G → A	G	172	V → I	3	19.1	0.52	0.61
	3742	A → G	F	393	I → V	1	17.8	0.52	0.50
	4493	A → G	G	171	T → A	8	17.7	0.35	0.49
	4494	C → T	G	171	T → I	2	16.9	0.59	0.43
	4011	A → G	G	10	N → S	4	16.4	0.40	0.38
	3587	C → T	F	340	A → V	1	16.2	0.30	0.30
	4951	G → A	H	142	G → D	1	14.1	0.37	0.19
NC13	4775	C → T	H	71	A → V	2	20.6	0.51	0.47
	3779	A → G	F	393	I → V	1	20.2	0.35	0.44
	4533	G → A	G	172	V → I	3	20.1	0.51	0.44
	4048	A → G	G	10	N → S	2	19.8	0.52	0.41
	4039	C → T	G	7	S → F	1	18.6	0.48	0.33
	3408	A → G	F	269	N → S	1	18.6	0.43	0.33
	4404	A → G	G	129	T → A	6	18.1	0.38	0.29
	4531	C → T	G	171	T → I	4	16.7	0.45	0.19
WA13	5238	G → C	H	50	A → P	1	21.5	0.33	0.64
	3702	C → T	F	203	T → I	7	21.1	0.29	0.60
	5299	C → T	H	70	T → M	1	21.0	0.37	0.59
	3234	C → T	F	47	A → V	3	20.5	0.38	0.56
	4048	G → T	F	319	L → F	1	20.1	0.25	0.53
	3951	C → T	F	287	A → V	1	19.8	0.28	0.50
	5289	C → T	H	67	P → S	2	19.8	0.32	0.50
	3401	G → C	F	104	D → H	2	18.9	0.30	0.44
	4023	C → T	F	311	T → I	1	18.6	0.28	0.41
	2962	C → T	D	148	A → V	1	17.1	0.35	0.30

^aFitness measured in log₂ doublings per hour.

^bSelection coefficient relative to wild-type fitness.