FIG 3 .

The MAb MN86-994-11-1 binding epitope is conserved among subfamily A and B fHBP variants present in the strain pool (n = 1,814). Peptide sequences of the MN86-994-11-1 K180-to-L198 binding site in subfamily A and B antigens defined by deuterium exchange mapping (Fig. 2) are compared. The number of variants represented by each sequence is indicated along with the mean MFI for each variant example and its corresponding frequency in the strain pool. An fHBP-B01 isolate (asterisk) is absent from the collection, but the epitope (shared with fHBP-B44) is included to permit comparisons with other fHBP variants for MAb MN86-994-11 binding affinity (see Table 1).