FIG 7 .

NmB fHBP surface expression level (MFI) and susceptibility to killing in an hSBA by bivalent fHBP immune serum. fHBP surface expression is determined by the binding of broadly cross-reactive MAb MN86-994-11-1 to NmB isolates in the MEASURE assay. The 109 NmB isolates were also tested in the hSBA with pooled human pre- and postvaccination bivalent rLP2086 immune serum samples. (A) For each of the 109 isolates, the postvaccination hSBA titer is plotted versus the level of fHBP surface expression. In cases where more than one assay was conducted with the same strain, the GMT was used. The symbol associated with each isolate conveys both the fHBP subfamily expressed and whether or not the isolate is killed (>4-fold increase in titer comparing pre- and postvaccination serum pools) with immune serum samples in an hSBA (B) Isolates were ordered from high to low fHBP surface expression and separated into killed and not killed groups on the basis of the ≥4-fold rise in hSBA titers between pre- and postvaccination serum pools. Green diamonds represent killed isolates, and red triangles represent isolates that were not killed. The dotted line marks the MFI threshold of 1,000.