TABLE 1 .
MAb MN86-994-11 binds with high affinity (subnanomolar KD) to both subfamily A and B fHBP variantsa
| fHBP variant (% identity) |
Value (error) |
||
|---|---|---|---|
| KD (M) | kon (1/Ms) | koff (1/s) | |
| A05 (100) | 2.29E-11 (<1E-12) | 3.02E+5 (2.77E+2) | 6.92E-6 (6.52E-8) |
| A12 (85) | 1.73E-11 (<1E-12) | 4.09E+5 (1.27E+3) | 7.06E-6 (1.94E-7) |
| A22 (89) | 8.64E-11 (<1E-12) | 2.07E+5 (2.22E+2) | 1.79E-5 (9.02E-8) |
| A29 (93) | 7.36E-11 (<1E-12) | 2.22E+5 (3.51E+2) | 1.63E-6 (1.37E-7) |
| B01 (100) | 5.73E-11 (<1E-12) | 3.12E+5 (2.28E+2) | 1.79E-5 (6.77E-8) |
| B09 (88) | 1.25E-11 (<1E-12) | 2.30E+5 (2.86E+2) | 2.88E-6 (1.08E-7) |
| B24 (87) | 2.58E-11 (<1E-12) | 2.57E+5 (3.30E+2) | 6.62E-6 (8.89E-8) |
| B44 (91) | 1.50E-10 (<1E-12) | 8.28E+5 (4.76E+3) | 1.24E-4 (5.95E-7) |
a
The affinity of MN86-994-11-1 for fHBP was determined with the Octet RED96 system. Four nonlipidated recombinant subfamily A variants (A05, A12, A22, and A29) and four nonlipidated subfamily B variants (B01, B09, B24, and B44) were tested. The percent amino acid sequence identities of fHBP variants to vaccine antigens A05 and B01 are indicated.