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. 2018 Mar 13;9(2):e00310-18. doi: 10.1128/mBio.00310-18

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Copyright © 2018 Rebelein et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — CO-reducing activities and product profiles of NifDK^M, VnfDGK^V, and VnfDGK^M. (A to C) Individual activities of hydrocarbon formation (A), distributions of hydrocarbon products (B), and total activities of hydrocarbon product formation (C) by NifDK^M, VnfDGK^V, and VnfDGK^M when CO is supplied as a substrate. Activities are expressed as nanomoles of product per nanomole of cofactor per minute in panels A and C. The percentage activities of proteins are shown in red in panel C, with the total activity of VnfDGK^V set at 100% and those of NifDK^M and VnfDGK^M calculated accordingly. Note that there is a contribution of V to the activity of VnfDGK^M due to the presence of <0.07 nmol of V per nmol of VnfDGK^M. However, the percentage contribution of V to the overall activity of VnfDGK^M cannot be conclusively determined due to the inaccuracy of V determination in this low concentration range.