. 2017 Jul 28;96(10):3676–3686. doi: 10.3382/ps/pex192

Table 6.

Jejunal expression of genes for nutrient transporters and digestive enzymes in broiler chickens fed corn-soybean meal based diets without or with epidermal growth factor and challenged with Eimeria

Treatments		Nutrient transporters³						Enzymes
Eimeria ¹	EGF², μg	NAA	Glut	CAT1	PEPT1	SGLT1	xCT1	Maltase	Sucrase
No	0	0.35	1.32^a	0.29^b	1.57	59.3^a	0.09^ab	4.59^ab	26.2^ab
No	80	0.28	1.09^ab	0.44^b	0.98	48.4^ab	0.16^ab	3.88^ab	17.3^ab
No	160	0.48	1.29^a	0.41^b	1.41	50.7^ab	0.21^ab	2.12^b	16.9^b
Yes	0	0.13	0.27^b	0.49^b	0.73	32.8^b	0.07^b	2.18^b	18.3^ab
Yes	80	0.77	1.09^ab	0.80^ab	2.11	40.2^ab	0.18^ab	4.32^ab	35.8^a
Yes	160	0.69	1.20^ab	1.26^a	1.64	57.0^a	0.40^a	6.15^a	33.5^ab
	SEM	0.25	0.32	0.23	0.54	8.41	0.10	1.07	6.37
Main effect, Eimeria
	No	0.37	1.23	0.47^b	1.32	52.8	0.16	3.53	20.1
	Yes	0.53	0.85	0.85^a	1.49	43.3	0.22	4.22	29.2
	SEM	0.15	0.22	0.15	0.31	4.86	0.06	0.62	3.68
Main effect, EGF, μg
	0	0.24	0.79	0.39	1.15	46.05	0.08^b	3.38	22.2
	80	0.52	1.09	0.62	1.54	44.29	0.17^ab	4.10	26.5
	160	0.58	1.24	0.83	1.52	53.82	0.30^a	4.14	25.2
	SEM	0.19	0.23	0.17	0.38	5.95	0.07	0.76	4.51
Probabilities
Eimeria	0.415	0.182	0.020	0.701	0.177	0.478	0.437	0.094
EGF	0.338	0.427	0.170	0.705	0.493	0.038^L	0.736	0.785
Eimeria*EGF	0.064	0.043	0.009^L	0.060	0.034	0.028	0.020^L	0.039^Q

¹Chicks were orally gavaged with a 1 mL mixture of 25,000 E. acervulina and 5,000 E. maxima on d 5, intestinal samples collected 5 (day 10 of life) d after challenge.

²Epidermal growth factor, μg/kg BW based on projected growth curve of Ross 708.

³NAA, neutral amino acids transporter; Glut, glutamate transporter; CAT1, cationic amino acids transporter; SGLT1, sodium dependent glucose transporter; PEPT1, Peptide transporter 1; xCT1, sodium independent Cys-Glu antiporter.

Means assigned different letters (a, b) within a factor of analysis (Eimeria, EGF and their interactions) are significantly different, P < 0.05.

^Llinear and ^Qquadratic effects of EGF.