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. 2018 Jan 30;10(2):58. doi: 10.3390/v10020058

Figure 6.

Figure 6

Neutralizing antibody responses induced by inactivated US/MO/14-18947 in adult mice. (A) SDS-PAGE and western blotting analysis of purified inactivated EVD68. Lane M, protein marker; lane Virus, inactivated US/MO/14-18947; (B) visualization of inactivated US/MO/14-18947 by electron microscopy; (C,D) serum antibody responses induced by inactivated US/MO/14-18947. Groups of six BALB/c mice were immunized twice with PBS (ctr) or inactivated US/MO/14-18947. Antisera were collected 2 weeks after the final immunization and used for different assays; (C) virus-specific antibody responses measured by ELISA with purified US/MO/14-18947 as the coating antigen. The antisera were diluted 1:1000; (D) neutralization titers against US/MO/14-18947. The antisera from PBS-immunized mice did not show any neutralization effect at the lowest dilution (1:16) and were assigned a titer of 8 for geometric mean titer (GMT) calculation. Each symbol represents a mouse, and the solid line indicates the geometric mean value of the group.